Fig 2: HLX expression in AML cell lines. (A) Comparison of HLX gene expression in different cell lines. (B) Comparison of HLX protein expression in different cell lines. (C) Gray value analysis of western blotting results. **P<0.01. NS, not significant; HLX, H2.0-like homeobox gene.

Fig 3: Flow cytometry analysis of the cell cycle distribution of NB4 cells transfected with siRNA-HLX2 for 24 h. (A) Negative control group. (B) Cells transfected with siRNA-HLX2 for 24 h. (C) Scatter plot of the negative control group. (D) Scatter plot of the cells transfected with siRNA-HLX2 for 24 h. (E) Cell cycle distribution. *P<0.05, **P<0.01. HLX, H2.0-like homeobox gene; siRNA, small interfering RNA; Neg, negative control.

Fig 4: Changes in HLX expression after siRNA transfection of NB4 cells. (A) HLX gene expression was determined by reverse transcription-quantitative PCR at 24 h after siRNA transfection. (B) HLX protein expression was detected by western blotting at 24 h after siRNA transfection. (C) Gray value analysis of western blotting results. **P<0.01. NS, not significant; HLX, H2.0-like homeobox gene; siRNA, small interfering RNA; Neg, negative control.

Fig 5: Changes in STAT5 protein expression in NB4 cells after siRNA-HLX2 transfection. (A) Comparison of STAT5 protein expression in NB4 cells after siRNA transfection. (B) Gray value analysis of western blotting results. *P<0.05, **P<0.01. NS, not significant; HLX, H2.0-like homeobox gene; siRNA, small interfering RNA; Neg, negative control.